CBLB502, an agonist of Toll-like receptor 5, has antioxidant and scavenging free radicals activities in vitro

详细信息    查看全文

• 作者：Weiguang Li^a ; ¹ ; Changhui Ge^a ; ¹ ; Liu Yang^a ; Ruixue Wang^a ; Yiming Lu^a ; Yan Gao^a ; Zhihui Li^a ; Yonghong Wu^a ; Xiaofei Zheng^a ; Zhaoyan Wang^b ; ^{2468li@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Chenggang Zhang^a ; ^{zhangcg@bmi.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Toll-like receptor 5 agonist ; CBLB502 ; Antioxidant ; Scavenging free radical ; Kinetics
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：97-103
• 全文大小：2161 K

文摘

The bacterial protein flagellin is the known agonist of Toll-like receptor 5 (TLR5). It has been reported that CBLB502, a novel agonist of TLR5 derived from Salmonella flagellin, could reduce radiation toxicity in mouse and primate models, protect mice from dermatitis and oral mucositis caused by radiation, inhibit acute renal ischemic failure, and inhibit the growth of A549 lung cancer cell. The property of CBLB502 is able to bind to TLR5 and activates NF-κB signaling. In this study, we investigated the antioxidant potential and free radicals scavenging properties of CBLB502 in vitro. Interestingly, we found that CBLB502 has a direct and distinct antioxidant capacity and can efficiently scavenge a variety of free radicals, including superoxide anion, hydroxyl radical, and ABTS cation (ABTS⁺). Through wave scanning and kinetic evaluation of scavenging ABTS⁺, we found that the ABTS⁺ scavenging process of CBLB502 is relatively slow, and the ABTS⁺ scavenging activity of CBLB502 has a consistently kinetics characteristics. In conclusion, our results suggested that CBLB502 has antioxidant and scavenging free radicals activities in vitro. It is implied that CBLB502 might partially promote the beneficial protective effect through its scavenging free radicals.