Comparative anti-psoriatic efficacy studies of clobetasol loaded chitin nanogel and marketed cream

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• 作者：Rajitha Panonnummal^a ; R. Jayakumar^b ; M. Sabitha^a ; ^{sabitham@aims.amrita.edu} ; ^{sabitha.mangalath@gmail.com}
• 关键词：APC ; antigen presenting cell ; DLS ; dynamic light scattering ; PFA ; paraformaldehyde ; COX ; cycloxygenase ; LOX ; lipoxygenase ; LPS ; lipo poly saccharide ; FBS ; fetal bovine serum ; IMQ ; imiquimod ; SOD ; superoxide dismutase ; MDA ; malon di-aldehyde ; GSH ; glutathione ; PASI ; Psoriatic Area Severity Index ; RPMI ; Rosewell Park Memorial Institute Medium
• 刊名：European Journal of Pharmaceutical Sciences
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：193-206
• 全文大小：2541 K
• 卷排序：96

文摘

In the present study chitin nanogel loaded with anti-psoriatic drug clobetasol was developed (CLCNG) for its topical delivery in psoriasis. CLCNG had the particle size of 132 ± 14 nm, with gel like consistency, stability in refrigerator, having higher drug release properties at acidic pH. CLCNG exhibited significant toxicity towards HaCaT and THP-1cell lines by MTT assay. The uptake of nanogel by HaCaT cell lines was confirmed by fluorescent microscopy. CLCNG at 0.35 mg/ml exhibited significant anti-inflammatory activity with an average of 65% and 70% inhibition in COX and LOX activities expressed in THP-1 cells. In vitro skin permeation studies revealed the increased transdermal flux with fragmented stratum corneum and loosened epidermal layers in CLCNG treated samples, compared with control drug solution. The in vivo anti-psoriatic studies done on imiquimod model confirmed the potential benefits of the nanogel for the topical delivery of clobetasol in psoriasis.