VEGF-A gene promoter polymorphisms and microvascular complications in patients with essential hypertension

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• 作者：Raffaele Palmirotta ; Patrizia Ferroni ; Giorgia Ludovici ; Francesca Martini ; Annalisa Savonarola ; Roberta D'Aless ; ro ; Valeria Raparelli ; Marco Proietti ; Antongiulio Scarno ; Silvia Riondino ; Stefania Ba
• 关键词：Essential hypertension ; VEGF-A ; VEGF-A polymorphisms
• 刊名：Clinical Biochemistry
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：43
• 期：13-14
• 页码：1090-1095
• 全文大小：544 K

文摘

Objectives

We investigated the possible involvement of vascular endothelial growth factor (VEGF-A) gene promoter polymorphisms in essential hypertension (EH).

Design and methods

1225 bp of the VEGF-A gene promoter were screened for polymorphisms using PCR amplification and direct DNA sequence analysis in 62 EH and 62 normotensive (HS) individuals. Circulating VEGF-A levels were determined by immunoassay.

Results

−152G/A (p = 0.009) and −116G/A (p = 0.016) polymorphisms were correlated to hypertension (p < 0.05). Median platelet VEGF-A load in EH was 2.10 fg/plt. Patients with microvascular complications (MC) had higher platelet VEGF-A load than those without (p = 0.005). Multivariate analyses showed that −116 A allele was an independent predictor of microalbuminuria (p = 0.014) and increased platelet VEGF-A load (p = 0.009) in EH. Platelet VEGF-A load independently predicted MC (p = 0.049) in addition to −116G/A polymorphism (p = 0.035).

Conclusions

Abnormal regulation of VEGF-A due to polymorphism at position −116 might represent a genetic factor for increased VEGF-A production and MC in EH.