Molecular snapshot of drug-resistant and drug-susceptible Mycobacterium tuberculosis

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• 作者：Violeta Valcheva ; Igor Mokrousov ; Olga Narvskaya ; Nalin Rastogi ; Nadya Markova
• 关键词：Drug resistance ; Mutation ; Spoligotyping ; VNTR
• 刊名：Infection, Genetics and Evolution
• 出版年：2008
• 出版时间：September 2008
• 年：2008
• 卷：8
• 期：5
• 页码：657-663
• 全文大小：777 K

文摘

We report results of the first study on the molecular basis of drug resistance in Mycobacterium tuberculosis strains currently circulating in Bulgaria. The study panel consisted of 133 (including 37 drug-resistant) isolates recovered from newly diagnosed, adult pulmonary TB patients from different regions of Bulgaria in 2005–2006. Three types of the rpoB mutations were found in 20 of 27 RIF-resistant isolates; rpoB S531L was the most frequent. Eleven (48 % ) of 23 INH-resistant isolates had katG S315T mutation. inhA −15C > T mutation was detected in one INH-resistant isolate (that also had katG315 mutation) and three INH-susceptible isolates. A mutation in embB306 was found in 7 of 11 EMB-resistant isolates. Comparison with spoligotyping and 24-VNTR locus typing data suggested that emergence and spread of drug-resistant and MDR-TB in Bulgaria are not associated with any specific spoligotype or MIRU-VNTR genotype.