Bone formation of human mesenchymal stem cells harvested from reaming debris is stimulated by low-dose bone morphogenetic protein-7 application in vivo
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- 作者：Fabian Westhauser ; Fabian.Westhauser@med.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author ; Melanie Hö ; llig ; Bruno Reible ; Kai Xiao ; Gerhard Schmidmaier ; Arash Moghaddam
- 关键词：Tissue engineering ; Human mesenchymal stem cells (MSC) ; Bone morphogenetic protein-7 (BMP-7) ; Micro-computed tomography (mCT) ; Reaming material
- 刊名：Journal of Orthopaedics
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：13
- 期：4
- 页码：404-408
- 全文大小：880 K
文摘
Stimulation of mesenchymal stem cells (MSC) by bone morphogenetic protein-7 (BMP-7) leads to superior bone formation in vitro. In this in vivo-study we evaluated the use of BMP-7 in combination with MSC isolated from reaming debris (RIA-MSC) and iliac crest bone marrow (BMSC) with micro-computed tomography (mCT)-analysis.
β-Tricalciumphosphate scaffolds coated with BMSC and RIA-MSC were stimulated with three different BMP-7-concentrations and implanted ectopically in severe combined immunodeficiency (SCID) mice.
Our results demonstrate that RIA-MSC show a higher osteogenic potential in vivo compared to BMSC. Ossification increased in direct correlation with the BMP-7-dose applied, however low-dose-stimulation by BMP-7 was more effective for RIA-MSC.