Identification of a soluble leptin receptor in crucian carp with different binding affinity to leptin-a and leptin-b

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• 作者：Feifei Xie¹ ; Xin Li¹ ; Saifan Huang ; Jiyuan Li ; Xiaopin Guo ; Yibin Cao ; ^{sky109@zjnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Crucian carp ; Soluble leptin receptor ; Leptin-a ; Leptin-b ; Teleost
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：108-111
• 全文大小：440 K

文摘

Soluble leptin receptor (sLepR) is the main leptin-binding protein in plasma and contributes to activation of circulating leptin. In this study, we identified a sLepR in plasma of crucian carp (Carassius carassius) using a pull-down assay, and the interaction of sLepR with its ligand is confirmed by a cross-linking study. In addition, we found that leptin-a has higher affinity than leptin-b for sLepR. According to our knowledge, this is the first experimental report about the main ligand of sLepR in teleost.