Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol
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- 作者：Jennifer E. Kaya ; b ; Michael C. Jewetta ; b ; c ; d ; m-jewett@northwestern.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cell-free metabolic engineering ; Cell-free synthetic biology ; 2 ; 3-butanediol ; Escherichia coli ; In vitro ; Cofactor regeneration
- 刊名：Metabolic Engineering
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：32
- 期：Complete
- 页码：133-142
- 全文大小：1196 K
文摘
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Highly active metabolic conversions were activated in a cell-free environment.
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Native glycolytic enzymes support a heterologous pathway in crude extracts.
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NADH is regenerated and recycled using native enzymes.
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Achieved 82 g L−1 titer with 11.3 g L−1 h−1 peak volumetric productivity in 30 h.