Asymmetric synthesis of lipitor chiral intermediate using a robust carbonyl reductase at high substrate to catalyst ratio
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- 作者：Guo-Chao Xu ; Ming-Hui Tang ; Ye Ni ; yni@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Asymmetric reduction ; Carbonyl reductase ; Ethyl (S)-4-chloro-3-hydroxybutanoate ; Substrate to catalyst ratio
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：67-72
- 全文大小：594 K
文摘
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RpCR shares low sequence identity with known COBE carbonyl reductases.
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RpCR exhibits a high kcat/Km of 1747 s−1 mM−1.
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The substrate to catalyst ratio and space-time yield of RpCR reached 70 and 1480 g L−1 d−1.