Activity improvement of a Kluyveromyces lactis aldo-keto reductase KlAKR via rational design
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- 作者：Xi Luoa ; b ; Ya-Jun Wanga ; b ; Wei Shena ; b ; Yu-Guo Zhenga ; b ; zhengyg@zjut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; zhengyg88320630@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aldo-keto reductase ; Atorvastatin calcium ; Rational engineering ; Site-saturation mutagenesis ; T-butyl 6-cyano-(3R ; 5R)-dihydroxyhexanoate
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 April 2016
- 年：2016
- 卷：224
- 期：Complete
- 页码：20-26
- 全文大小：1196 K
文摘
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Significantly enhanced the activity of KlAKR via rational design.
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Robust mutant for t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate synthesis was gained.
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The mechanism was elucidated by molecular docking.
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KlAKR-Y295W/W296L showed improved activity toward the tested carbonyl compounds.