A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.)
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- 作者：Cheng Chaohuaa ; c ; Zang Gonggua ; ibfczgg@sina.com" class="auth_mail" title="E-mail the corresponding author ; Zhao Lininga ; Gao Chunshenga ; Tang Qinga ; Chen Jianhuaa ; Guo Xinbob ; Peng Dingxiangc ; Su Jianguanga ; jgsu@vip.163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：PGR ; plant growth regulator ; BA ; N6-benzyladenine ; NAA ; a-naphthaleneacetic acid ; TDZ ; 1-phenyl-3-(1 ; 2 ; 3-thiadiazol-5-yl) urea (thidiazuron) ; THC ; tetrahydrocannabinol ; ZT ; zeatin ; THC ; tetrahydrocannabinol ; CBD ; cannabidiol ; IBA ; indole-3-butyric acid
- 刊名：Industrial Crops & Products
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：83
- 期：Complete
- 页码：61-65
- 全文大小：1012 K
文摘
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Cotyledons were used as explants in the rapid shoot regeneration method in hemp.
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TDZ applied in MS medium were found to be capable of inducing in vitro shoots from cotyledons superior than BA and ZT.
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Younger cotyledons (2–3 DAP) produced significantly higher regeneration frequency than elder ones (5–6 DAP).
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The regeneration method was suitable while partly genotype dependent.
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The method may afford an alternative method for micropropagation and germplasm conservation, and the in vitro plantlets may be suitable to set up a transformation system.