Neuronal progenitor transplantation and respiratory outcomes following upper cervical spinal cord injury in adult rats

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• 作者：Todd E. White ; Michael A. Lane ; Milapjit S. S ; hu ; Barbara E. O'Steen ; David D. Fuller ; Paul J. Reier
• 关键词：Rats ; Fetal spinal cord ; Ventilation ; Phrenic ; Transplantation ; Respiratory ; Cervical spinal cord injury
• 刊名：Experimental Neurology
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：225
• 期：1
• 页码：231-236
• 全文大小：1088 K

文摘

Despite extensive gray matter loss following spinal cord injury (SCI), little attention has been given to neuronal replacement strategies and their effects on specific functional circuits in the injured spinal cord. In the present study, we assessed breathing behavior and phrenic nerve electrophysiological activity following transplantation of microdissected dorsal or ventral pieces of rat fetal spinal cord tissue (FSC_D or FSC_V, respectively) into acute, cervical (C2) spinal hemisections. Transneuronal tracing demonstrated connectivity between donor neurons from both sources and the host phrenic circuitry. Phrenic nerve recordings revealed differential effects of dorsally vs. ventrally derived neural progenitors on ipsilateral phrenic nerve recovery and activity. These initial results suggest that local gray matter repair can influence motoneuron function in targeted circuits following spinal cord injury and that outcomes will be dependent on the properties and phenotypic fates of the donor cells employed.