Allergenicity evaluation of fragrance mix and its ingredients by using ex vivo local lymph node assay-BrdU endpoints

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• 作者：Ozge Cemiloglu Ulker ; Yesim Kaymak ; Asuman Karakaya
• 关键词：AAALAC ; Association for Assessment and Accreditation of Laboratory Animal Care ; AOO ; Acetone ; Olive Oil ; EC3 ; estimated concentration ; FBS ; Fetal Bovine Serum ; ICCVAM ; Interagency Coordinating Committee on the Validation of Alternative Methods ; IFN ; interferon ; IL ; interleukin ; LLNA ; Local Lymph Node Assay ; PHA-L ; phytohemagglutinin-L ; SI ; Stimulation Index
• 刊名：Food and Chemical Toxicology
• 出版年：March, 2014
• 年：2014
• 卷：65
• 期：Complete
• 页码：162-167
• 全文大小：1543 K

文摘

The present studies were performed to compare the differences between sensitization potency of fragrance mix and its ingredients (oak moss absolute, isoeugenol, eugenol, cinnamal, hydroxycitronellal, geraniol, cinnamic alcohol, alpha amyl cinnamal), by using ex vivo LLNA-BrdU ELISA. The SI and EC₃ values were calculated and potency classification was found for the mixture and for each ingredients. T_H1 cytokines (IL-2, IFN-纬) and T_H2 cytokines (IL-4, IL-5) releases from lymph node cell culture were also investigated as contact sensitization endpoints. The EC₃ values were calculated and the potency of contact sensitization were classified for fragrance mix, oak moss absolute, isoeugenol, eugenol, cinnamal, hydroxycitronellal, geraniol, cinnamic alcohol, alpha amyl cinnamal respectively: 4.4% (moderate), 3.4% (moderate), 0.88% (strong), 16.6% (weak), 1.91% (moderate), 9.77% (moderate), 13.1% (weak), 17.93% (weak), 7.74% (moderate). According to our results it should be concluded that exposure to fragrance mix does not constitute an evidently increased hazard compared to exposure to each of the eight fragrance ingredients separately. Cytokine analyses results indicate that both T_H1 and T_H2 cytokines are involved in the regulation of murine contact allergy and can be considered as useful endpoints.