Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain
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- 作者：Matthew A. Stetz ; Marie V. Carter ; A. Joshua Wand ; wand@upenn.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lac repressor ; NMR spectroscopy ; tetA promoter ; Protein deuteration
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：75-82
- 全文大小：1011 K
文摘
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Optimized expression system and purification of recombinant Lac repressor is described.
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Biophysical quantities of isotopically enriched protein for NMR are accessible.
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Lac repressor regulatory domain can be expressed without toxicity in high yield.
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Solution NMR confirms Lac proteins are highly homogeneous and functional.