Hyaluronan production and molecular weight is enhanced in pathway-engineered strains of lactate dehydrogenase-deficient Lactococcus lactis
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- 作者：Mandeep Kaur1 ; Guhan Jayaraman ; guhanj@iitm.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hyaluronic acid molecular weight ; Lactococcus lactis ; Lactate dehydrogenase mutant ; NAD+/NADH ratio ; HA-precursor concentration ratio ; Transcriptional regulator Rex
- 刊名：Metabolic Engineering Communications
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：3
- 期：Complete
- 页码：15-23
- 全文大小：1254 K
文摘
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Hyaluronan MW and production is higher in engineered ldh-mutant L. lactis strains.
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Excess ethanol production in ldh-L. lactis cultures increased the NAD+/NADH ratio.
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The initial NAD+/NADH ratio showed a positive correlation with HA molecular weight.
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The NAD+/NADH ratio showed a positive correlation with the HA-precursor ratio.
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Rex-repressor regulates transcription of genes in HA and other metabolic pathways.