Molecular diagnostics for detecting pyrethroid and abamectin resistance mutations in Tetranychus urticae

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• 作者：Aris Ilias^a ; Vassilis A. Vassiliou^b ; John Vontas^c ; ^d ; Anastasia Tsagkarakou^a ; ^{tsagkarakou@nagref.gr}
• 关键词：Mite ; Bioassay ; Resistance management ; Monitoring ; Genotype
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：9-14
• 全文大小：1019 K
• 卷排序：135

文摘

Taqman assays were developed and used to monitor abamectin (G314D, G326E) and pyrethroid (F1538I) resistance mutations The frequency of resistance mutations largely varied among T. urticae from Greece and Cyprus, in accordance with pesticide use TaqMan diagnostics showed higher resolution in detecting alleles in low frequency, compared to massive sequencing approaches