Gastroprotective effect and chemical characterization of a polysaccharide fraction from leaves of Croton cajucara

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• 作者：Adamara M. Nascimento^a ; ^b ; Daniele Maria-Ferreira^c ; Evana Figueiredo J. de Souza^c ; Lauro M. de Souza^a ; ^d ; Guilherme L. Sassaki^a ; Marcello Iacomini^a ; Maria Fernanda de P. Werner^c ; ^{mfernanda.werner@ufpr.br} ; Thales R. Cipriani^a ; ^{trcipriani@ufpr.br}
• 关键词：Croton cajucara ; Polysaccharide ; Antiulcer activity
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：153-159
• 全文大小：1349 K
• 卷排序：95

文摘

Croton cajucara Benth. is a tree from the Amazon Forest, where it is known as sacaca. Its leaves and barks are used in medicinal preparations to treat different diseases, including gastric ulcers. The crude polysaccharide fraction (CCP), obtained from the hot aqueous extract of C. cajucara leaves, was able to promote gastroprotection on an ethanol induced gastric ulcer model. Therefore, a bioguided fractionation was performed to isolate the active polysaccharide fraction. After freezing-thawing, ultrafiltration and dialyses at 100, 50, and 25 kDa cut-off membranes, fraction 25R was obtained. It contained glucose, galactose, rhamnose, arabinose, galacturonic acid and mannose in a 7:5:5:3:1:1 molar ratio approximately, and had a Mw of 42,840 g/mol. Methylation analysis and NMR spectroscopy indicated that 25R is a very complex polysaccharide fraction containing type I rhamnogalacturonan, arabinan, type I arabinogalactan, type II arabinogalactan, rhamnan, starch and mannan. It was able to reduce ethanol-induced gastric ulcers in rats, through preservation of mucus and GSH levels.