Polysaccharides with immunomodulating properties from the bark of Parkia biglobosa
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- 作者：Yuan-Feng Zou ; Bing-Zhao Zhang ; Kari Tvete Inngjerdingen ; Hilde Barsett ; Drissa Diallo ; Terje Einar Michaelsen ; Elnour El-zoubair ; Berit Smestad Paulsen
- 关键词：AG-I ; arabinogalactan type I ; AG-II ; arabinogalactan type II ; Ara ; arabinose ; Gal ; galactose ; GalA ; galacturonic acid ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectrometry ; Glc ; glucose ; GlcA ; glucuronic acid ; LPS ; lipopolysaccharide ; Man ; mannose ; NO ; nitric oxide ; RG-I ; rhamnogalacturonan type I ; RG-II ; rhamnogalacturonan type II ; Rha ; rhamnose ; Xyl ; xylose ; 4-OMe-GlcA ; 4-O-methyl-glucuronic acid
- 刊名：Carbohydrate Polymers
- 出版年：30 January, 2014
- 年：2014
- 卷：101
- 期：Complete
- 页码：457-463
- 全文大小：696 K
文摘
The bark of Parkia biglobosa is used in traditional medicine to cure a wide range of illnesses. Polysaccharides were extracted from the bark with 50% ethanol-water, 50 掳C and 100 掳C water, and seven active fractions obtained by anion exchange chromatography and gel filtration. The complement fixation and macrophage stimulating activities of the different fractions were determined. The acidic fractions PBEII-I and PBEII-IV were the most active in the complement fixation assay, but the other fractions were also potent compared to the positive control BPII from Biophytum petersianum. Fractions PBEII-I and PBEII-IV were also the most potent fractions in stimulating macrophages to release nitric oxide. Structural studies showed that PBEII-I and PBEII-IV were pectic type polysaccharides, containing arabinogalactan type II structures. The observed differences in biological activities among the seven purified polysaccharide sub-fractions are probably due to differences in monosaccharide compositions, linkage types and molecular sizes.