A Rhizobium sp. TH capable of degrading MCLR was isolated for the first time.
The isolate degrades MCLR via an mlr-dependent mechanism.
mlrA arose in α-proteobacteria and was transferred to two β- and γ-proteobacteria.
mlrA exists mainly in α-proteobacteria and is seldom present in other bacteria.
A pair of primers was designed to target the mlrA gene.