Coating lanthanide nanoparticles with carbohydrate ligands elicits affinity for HeLa and RAW264.7 cells, enhancing their photodamaging effect

详细信息    查看全文

• 作者：Takashi Kanamori^a ; Takashi Sawamura^a ; Tatsumi Tanaka^a ; Izumi Sotokawa^a ; Ryota Mori^a ; Kotaro Inada^a ; Akihiro Ohkubo^a ; Shun-Ichiro Ogura^a ; Yasutoshi Murayama^b ; Eigo Otsuji^b ; Hideya Yuasa^a ; ^{hyuasa@bio.titech.ac.jp}
• 关键词：DIC ; 1 ; 3-diisopropylcarbodiimide ; DIPEA ; N ; N-diisopropylethylamine ; DLS ; dynamic light scattering ; DMAP ; N ; N-dimethyl-4-aminopyridine ; DMF ; N ; N-dimethylformamide ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; EPR effect ; enhanced permeability and retention effect ; HATU ; 1-[bis(dimethylamino)methylene]-1H-1 ; 2 ; 3-triazolo[4 ; 5-b]pyridinium 3-oxid hexafluorophosphate ; HOBt ; 1-hydroxybenzotriazole ; MTT ; 3-[4 ; 5-dimethylthiazol-2-yl]-2 ; 5-diphenyltetrazolium bromide
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：25
• 期：2
• 页码：743-749
• 全文大小：1018 K
• 卷排序：25

文摘

Lanthanide nanoparticles (LNPs) conjugated with monosaccharides were synthesized as a photon energy-upconverting nanodevice with affinity to cancer cells. The conjugates were designed to selectively damage the cancer cells containing protoporphyrin IX, a photosensitizer endogenously synthesized from priorly administrated 5-aminolevlunic acid (ALA), by a highly tissue-penetrative near-infrared (NIR) irradiation. First of all, the affinities of monosaccharides toward cells (HeLa, RAW264.7, and MKN45) were assessed by a novel cell aggregation assay with trivalent monosaccharide-citric acid conjugates. As a result, HeLa exhibited high affinity for glucose, while RAW264.7 for glucose, galactose, mannose, and fucose. A similar cell-monosaccharide affinity was microscopically observed when the cells were mixed with monosaccharide-LNP conjugates and rinsed, in which the high affinity LNP probes luminesced on the cells. The high affinity monosaccharide-LNPs showed greater photodamaging effects than the unmodified LNP toward the corresponding cells, when the cells were pretreated with ALA and irradiated by NIR. This study demonstrates that carbohydrates can be used as selective ligands for cancer cells in a photodynamic therapy with LNP.