The new antigen rKLO8 from an African strain of L. donovani was tested for diagnosis of CVL. The rKLO8 protein is suitable for diagnosis of CVL, with a similar performance compared to the reference antigen rK26. Combination of rKLO8 and rK26 shows improved diagnostic performance of CVL. Inclusion of rKLO8 in the DPP® rapid test deserves further attention.