A freely available semi-automated method for quantifying retinal ganglion cells in entire retinal flatmounts
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- 作者：E. Geeraertsa ; E. Dekeystera ; D. Gaublommeb ; c ; M. Salinas-Navarroa ; L. De Groefa ; L. Moonsa ; lieve.moons@bio.kuleuven.be" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mouse ; Glaucomatous optic neuropathies ; Retinal ganglion cell ; Semi-automated quantification ; Brn3a ; ImageJ
- 刊名：Experimental Eye Research
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：147
- 期：Complete
- 页码：105-113
- 全文大小：1552 K
文摘
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The developed ImageJ script counts Brn3a-immunopositive retinal ganglion cells (RGCs) in a retinal flatmount.
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The results of the script completely agree with data from manual counting.
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The script can successfully assess RGC degeneration in different rodent models of glaucoma.
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The script is an easily accessible tool to accurately quantify RGCs in an objective manner.
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Because it only relies on a few parameters, the script can easily be optimized for other labeling methods in various tissues.