Large-Scale Analysis Exploring Evolution of Catalytic Machineries and Mechanisms in Enzyme Superfamilies
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- 作者：Nicholas Furnham1 ; &dagger ; ; ; Natalie L. Dawson2 ; &dagger ; ; Syed A. Rahman3 ; Janet M. Thornton3 ; &Dagger ; ; Christine A. Orengo2 ; &Dagger ;
- 关键词：EC ; Enzyme Commission ; IUBMB ; International Union of Biochemistry and Molecular Biology ; CSA ; Catalytic Site Atlas ; SSG ; structurally similar groups ; TIM ; triosephosphate isomerase ; CMSS ; Catalytic Machinery Similarity Score ; NP ; normalised proportion ; GOX ; glycolate oxidase ; FCB ; flavocytochrome b2 ; FMN ; flavin mononucleotide
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：428
- 期：2
- 页码：253-267
- 全文大小：1388 K
文摘
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Examining how enzyme function evolves using sequence, structure, and reaction mechanism data.
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Quantifying changes in reaction mechanisms reveals how function has diverged in many superfamilies.
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Homologous domains frequently use different catalytic residues, which sometimes perform the same enzyme chemistry.
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This large-scale analysis has significance in protein function prediction and enzyme design.