- 作者：Pâ ; mela Letí ; cia dos Santos&lowast ; ; pamelalsantos@hotmail.com ; Thallita Pereira Queiroz ; DDS ; MSc ; PhD&dagger ; ; Rogé ; rio Margonar ; DDS ; MSc ; PhD&Dagger ; ; Abrahã ; o Cavalcante Gomes de Souza Carvalho ; DDS ; MSc§ ; ; Roberta Okamoto ; DDS ; MSc ; PhD|| ; Ana Paula de Souza Faloni ; DDS ; MSc ; PhD¶ ; ; Idelmo Rangel Garcia Jú ; nior ; DDS ; MSc ; PhD#
- 刊名：Journal of Oral and Maxillofacial Surgery
- 出版年：2013
- 出版时间：March, 2013
- 年：2013
- 卷：71
- 期：3
- 页码：505-512
- 全文大小：1596 K
Purpose
To evaluate the effect of implant osteotomy on immediate bone cell viability, comparing guided surgery for implant placement with the classic drilling procedure.Materials and Methods
For this study, 20 rabbits were used. The animals were divided into a guided surgery group (GG) and a control group (CG) and were then divided into 4 subgroups¡ªsubgroups 1, 2, 3, and 4¡ªcorresponding to drills used 10, 20, 30, and 40 times, respectively. All animals received 5 osteotomies in each tibia, by use of the classic drilling procedure in one tibia and guided surgery in the other tibia. The osteotomized areas were removed and processed immunohistochemically for detection of osteocalcin, receptor activator of nuclear factor ¦ÊB ligand (RANKL), osteoprotegerin (OPG), and caspase?3.
Results
Immunohistochemical analysis showed that osteocalcin expression was initially higher in the CG and remained constant after drill reutilization. Although the expressions of RANKL and OPG were not statistically different for the GG and CG, the RANKL/OPG ratio tended to be higher for the GG. Moreover, caspase 3 expression was elevated in the GG, proportionally to the number of osteotomies, indicating an increase in the apoptosis index in the GG.
Conclusions
The classic drilling procedure is more favorable to cell viability than guided surgery.