Nucleotide sequencing and phylogenetic analysis of the 3¡ä region of glycoprotein C gene of South American bovine herpesviruses 1 and 5

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• 作者：Carolina Kist Traesel^a ; Mariana S&#225 ; e Silva^b ; Fernando Rosado Spilki^c ; Rudi Weiblen^a ; Eduardo Furtado Flores^a ; ^{eduardofurtadoflores@gmail.com}
• 关键词：BoHV-1 ; BoHV-5 ; gC ; Transmembrane helix ; Cytoplasmic tail
• 刊名：Research in Veterinary Science
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：94
• 期：1
• 页码：178-185
• 全文大小：976 K

文摘

We herein describe a molecular analysis based on the 3¡ä region of the glycoprotein C gene of 45 bovine herpesvirus (BoHV) isolates from Brazil (n = 41), Uruguay (n = 2) and Argentina (n = 2). Nucleotide (nt) sequencing and alignment of 333 nt revealed levels of similarity ranging from 99.1 % to 100 % among BoHV-1 sequences (n = 12); 96.2-100 % among BoHV-5 sequences (n = 32); and 77.7-90.3 % between BoHV-1 and BoHV-5 sequences. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The isolate SV 453/93 (BoHV-1 associated with genital disease) could not be included within BoHV-1 subtypes since it presented a markedly distinct nt and amino acid (aa) deduced sequences. A transmembrane domain of 24 aa and the putative cytoplasmic tail of 8 aa were identified and mapped. These results indicate that this genome region represents a suitable target for phylogenetic subgrouping of BoHV-1 and BoHV-5 isolates and, perhaps, for understanding evolutionary relationships.