Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes - A new assay suitable for bench and bedside conditions
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- 作者：Tiago Granja ; tiago.granja@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Jessica Schad jessica.schad@student.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Patricia Schü ; ssel patricia.schuessel@student.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Claudius Fischer Claudius.Fischer@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Helene Hä ; berle Helene.Haeberle@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Peter Rosenberger peter.rosenberger@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author ; Andreas Straub andreas.straub@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：ab ; antibody ; ADP ; Adenosine diphosphate ; APC ; Allophycocyanin Conjugate ; BV421 ; Brilliant Violet 421 ; CD ; Cluster of Differentiation ; COSIMA ; Coagulation and Systemic Inflammation During Mechanical Circulatory Assist ; ECC ; Extracorporeal circulation ; ECLS ; Extracorporeal life support ; ECMO ; Extracorporeal membrane oxygenation ; FITC ; Fluorescein isothiocyanate ; FSC ; Forward scatter ; GP ; Glycoprotein ; HIT ; Heparin-induced thrombocytopenia ; LIBS ; Ligand induced binding site ; LPS ; Lipopolysacharide
- 刊名：Thrombosis Research
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：136
- 期：4
- 页码：786-796
- 全文大小：1311 K
文摘
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Platelet leukocyte complexes are key mediators in thromboinflammatory disorders.
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We describe a new flow cytometry assay to analyze platelet leukocyte interaction.
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Platelet and leukocyte activation is measured using a six-colour approach.
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The sensitivity of the assay after stimulation with various agonists is shown.
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The applicability of this assay for bench and bedside analyses is demonstrated.