Impedance-based analysis of mu opioid receptor signaling and underlying mechanisms
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- 作者：David Thirkettle-Watts David.Thirkettle-Watts@dsto.defence.gov.au" class="auth_mail" title="E-mail the corresponding author
- 关键词：MOR ; mu opioid receptor ; GPCR ; G-protein coupled receptor ; CI ; cell index ; RTCA ; real-time cellular analysis ; CHO ; Chinese hamster ovary ; FCS ; foetal calf serum ; DAMGO ; [D-Ala2 ; NMe-Phe4 ; Gly-ol5]-enkephalin
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：6
- 期：Complete
- 页码：32-38
- 全文大小：2024 K
文摘
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An impedance assay was used to capture label-free real-time data for two opioids.
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DAMGO and morphine treatments produced different responses.
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Cellular mechanisms underlying impedance response were investigated.
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G-protein signaling and protein phosphorylation were implicated in the response.
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The contribution of two kinases, AKT1/2/3 and ERK1/2, was demonstrated.