Identification of antigen-specific human monoclonal antibodies using high-throughput sequencing of the antibody repertoire
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- 作者：Ju Liu ; Ruihua Li ; Kun Liu ; Liangliang Li ; Xiaodong Zai ; Xiangyang Chi ; Ling Fu ; Junjie Xu ; xujunjie@sina.com" class="auth_mail" title="E-mail the corresponding author ; Wei Chen ; cw0226@foxmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：High-throughput sequencing ; Antibody repertoire ; Antigen-specific human monoclonal antibodies ; Screening strategy
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：22 April 2016
- 年：2016
- 卷：473
- 期：1
- 页码：23-28
- 全文大小：535 K
文摘
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NGS was used to screen the human antibody repertoire following vaccination.
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IgH and IgK CDR3 frequency and dynamics were assessed simultaneously.
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Clones were predicted as positive or negative based on frequency and dynamics.
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The screening strategy achieved a positive rate of more than 50%.
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The screening efficiency was improved compared with previously reported methods.