Integration of target responsive hydrogel with cascaded enzymatic reactions and microfluidic paper-based analytic devices (µPADs) for point-of-care testing (POCT)
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- 作者：Tian Tiana ; 1 ; Xiaofeng Weib ; 1 ; Shasha Jiaa ; Ruihua Zhanga ; Jiuxing Lia ; Zhi Zhua ; zhuzhi@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Huimin Zhanga ; Yanli Maa ; Zhenyu Linb ; Chaoyong James Yanga ; cyyang@xmu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：µ ; PAD ; Hydrogel ; Point-of-care ; Aptamer ; Cascaded enzymatic reactions
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 March 2016
- 年：2016
- 卷：77
- 期：Complete
- 页码：537-542
- 全文大小：1963 K
文摘
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We constructed a hydrogel–μPAD system for POCT.
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A glucoamylase-trapped aptamer-crosslinked hydrogel was used for molecular recognition.
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Cascade enzymatic reactions were integrated for signal amplification
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The system allows semiquantitation by naked eyes and quantitation by handheld devices
µPADs were used for portable readout.