Engineering degrons of yeast ornithine decarboxylase as vehicles for efficient targeted protein degradation
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- 作者：Rushikesh G. Joshi ; Swapnali Kulkarni ; C. Ratna Prabha ; chivukula_r@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：ODC ; ornithine decarboxylase ; AzBE ; antizyme binding element ; yEGFP ; yeast enhanced green fluorescent protein ; TFE ; 2 ; 2 ; 2-trifluoroethanol
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：1850
- 期：12
- 页码：2452-2463
- 全文大小：1713 K
文摘
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Two degrons in yeast ornithine decarboxylase sequence target it for degradation.
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They are N-terminal fifty residues and 伪/尾 domain with antizyme binding sequence.
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Three peptides N50, 伪/尾 and N伪/尾 were examined for their degradation potentials.
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N伪/尾 regulated by antizyme is a better degron than N50, while 伪/尾 failed as a degron.
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Functional efficacy of the degrons is a consequence of their structural integrity.