Immunome and venome of Bothrops jararacussu: A proteomic approach to study the molecular immunology of snake toxins

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• 作者：Carlos Correa-Netto ; Ricardo Teixeira-Araujo ; Aniesse Silva Aguiar ; Aní ; bal Rafael Melgarejo ; Salvatore Giovanni De-Simone ; Má ; rcia Regina Soares ; Debora Foguel ; Russolina Benedeta Zingali
• 关键词：Bothrops jararacussu ; Bothrops jararaca ; Crotalus durissus ; Snake venom proteome ; Immunome
• 刊名：Toxicon
• 出版年：2010
• 出版时间：15 June 2010
• 年：2010
• 卷：55
• 期：7
• 页码：1222-1235
• 全文大小：2816 K

文摘

A combination of anti-bothropic and anti-crotalic sera has been reported to be more effective in neutralizing the effects of Bothrops jararacussu venom than anti-bothropic serum alone. The role of proteins from B. jararacussu venom in the horse immune response was evaluated via the analysis of cross-reactivity with homologous and heterologous sera. Many of the proteins in B. jararacussu venom were identified via 2D gel electrophoresis. Western blots revealed that anti-jararacussu showed higher reactivity to l-aminoxidase (LAOs) and snake venom metalloproteinase, (SVMPs) and weaker reactivity towards Snake venom serine proteases (SVSPs), PLAb>2b>, C-type lectin and cysteine-rich proteins. Anti-jararaca preferentially recognized LAOs, SVMPs and SVSPs. Both of these sera failed to recognize low-molecular weight proteins. Anti-crotalic serum clearly recognized LAOs, C-type lectin, SVSP, cysteine-rich proteins, SVMP and Asp49-PLAb>2b>. The cross-reactivity with anti-PLAb>2b> revealed the immunoreactivity of these antibodies to proteins with molecular masses in a range that is poorly recognized by other studied anti-sera. Our results suggest that the contribution of anti-crotalic serum to the neutralization of B. jararacussu by may be due to its cross-reactivity with proteins such as C-type lectins, SVSPs, Asp49-PLAb>2b>. These results also reinforce the importance of neutralizing the highly toxic proteins inclusive those with low immunogenicity in commercial antivenom production to obtain a highly protective serum against snake venoms.