Abnormal movement of tropomyosin and response of myosin heads and actin during the ATPase cycle caused by the Arg167His, Arg167Gly and Lys168Glu mutations in TPM1 gene
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- 作者：Yurii S. Borovikova ; borovikov@incras.ru" class="auth_mail" title="E-mail the corresponding author ; Nikita A. Ryseva ; Aleksey A. Cherneva ; Stanislava V. Avrovaa ; Olga E. Karpichevaa ; Danuta Borysb ; Małgorzata Śliwińskab ; Joanna Moraczewskab
- 关键词：Tropomyosin ; F-actin ; Myosin ; Troponin ; Ghost muscle fibres ; Congenital myopathies ; Fluorescence polarization
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：606
- 期：Complete
- 页码：157-166
- 全文大小：1449 K
文摘
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Effects of Arg167His, Arg167Gly and Lys168Glu mutations in tropomyosin were studied.
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Polarized fluorescence reported orientation of tropomyosin, actin and myosin heads.
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Mutant tropomyosins were shifted from the normal position on the actin filament.
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Arg167His decreased but Arg167Gly and Lys168Glu increased the switching on of actin monomers.
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Arg167Gly or Lys168Glu increased fraction of myosin bound in rigor.