Spectrofluorimetric determination of fexofenadine hydrochloride in pharmaceutical preparation using silver nanoparticles

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• 作者：Zeid Abdullah Alothman ; Nausheen Bukhari ; Sajjad Haider ; Saikh Mohammad Wabaidur ; Abdulrahman A. Alwarthan
• 关键词：Fexofenadine hydrochloride ; Silver nanoparticles ; Fluorescence ; Quenching
• 刊名：Arabian Journal of Chemistry
• 出版年：2010
• 出版时间：October 2010
• 年：2010
• 卷：3
• 期：4
• 页码：251-255
• 全文大小：650 K

文摘

A novel sensitive fluorimetric method was investigated for the assay of fexofenadine hydrochloride (FEX) using silver nanoparticles (NPs) as a fluorescence probe. The NPs, which were prepared by chemical reduction of silver nitrate with sodium borohydride (reducing agent) in aqueous solution (without organic stabilizers) were water soluble, stable and had narrow emission band. The addition of drug to NPs solution caused considerable quenching of the emission band of silver NPs, which was likely due to the complexation of the drug to silver NPs. Under the optimum conditions, the quenched fluorescence (FL) intensity was linear with the concentration of FEX in the range of 1 × 10⁻⁷ to 2.5 × 10⁻⁵ mol L⁻¹ (0.9985) with a detection limit of 1.2 × 10⁻⁸ mol L⁻¹. The quenching mechanism of the studied drug on the emission band of silver NPs was explained by Stern–Volmer law. The developed method was applied to FEX determination in a pharmaceutical formulation (allegra tablets) and biological fluids (human serum and urine).