Golden dorado (Salminus brasiliensis) is an increasingly popular catch-and-release fish species in South America. This study provides insight into best practices development for this previously unevaluated recreational fishery. Notable correlations were physiological stress with air exposure and handling, and fallback at release with reflex impairment. Short-term radio tag relocations (42-day tracking period) suggested a maximum 8% mortality. Recreational anglers can reduce the consequences of catch-and-release on golden dorado by minimizing air exposure and reducing handling times.