CHI3L1 nuclear localization in monocyte derived dendritic cells

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• 作者：Michelino Di Rosa^a ; ¹ ; ^{mdirosa@unict.it" class="auth_mail" title="E-mail the corresponding author} ; Daniele Tibullo^b ; Salvatore Saccone^c ; Gisella Distefano^a ; Maria Sofia Basile^a ; Francesco Di Raimondo^b ; Lucia Malaguarnera^a
• 关键词：CHI3L1 and YKL40 ; chitinase 3 like 1 ; moDCs ; monocyte derived dendritic cells ; iDCs ; immature dendritic cells ; mDCs ; mature dendritic cells ; GM-CSF ; granulocyte-macrophage colony-stimulating factor ; IL-4 ; interleukin-4 ; LPS ; lipopolysaccharide ; IFN-γ ; interferon-γ ; CCL2 ; the chemokine (C&ndash ; C motif) ligand 2 ; CXCL2 ; chemokine (C&ndash ; X&ndash ; C motif) ligand 2 ; MMP-9 ; matrix metallopeptidase 9 ; IL6 ; interleukin-4 ; M1 ; macrophages M1
• 刊名：Immunobiology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：221
• 期：2
• 页码：347-356
• 全文大小：4056 K

文摘

Chitinase-3-like-1 protein (CHI3L1) is a glycosyl hydrolase (GH) highly expressed in a variety of inflammatory diseases at infectious and non-infectious etiology. CHI3L1 is produced by a wide variety of cells including monocyte-derived macrophages cell lines such as polarized M1 and M2 type macrophages, osteoclasts and Kupffer cells. In this study we have examined the expression of CHI3L1 during the differentiation and maturation of dendritic cells. Magnetically-isolated peripheral blood monocytes were differentiated toward immature DCs (iDC) and mature DCs (mDCs) through a combination of factors and cytokines. Our result showed, for the first time, that CHI3L1 is expressed during the process of differentiation and maturation of dendritic cells in time dependent manner. Furthermore, the CHI3L1 is evenly distributed in cytoplasm and in the nucleus of both the iDCs and mDCs. These results suggest that CHI3L1 may play crucial role in the DCs immunoresponse.