Determining the optimal number of individual samples to pool for quantification of average herd levels of antimicrobial resistance genes in Danish pig herds using high-throughput qPCR
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- 作者：Julie Clasena ; jucl@vet.dtu.dk" class="auth_mail" title="E-mail the corresponding author ; Anders Mellerupa ; amellerup84@gmail.com" class="auth_mail" title="E-mail the corresponding author ; John Elmerdahl Olsenb ; jeo@sund.ku.dk" class="auth_mail" title="E-mail the corresponding author ; Ø ; ystein Angenc ; ysan@ssi.dk" class="auth_mail" title="E-mail the corresponding author ; Anders Folkessona ; afol@vet.dtu.dk" class="auth_mail" title="E-mail the corresponding author ; Tariq Halasaa ; tahbh@vet.dtu.dk" class="auth_mail" title="E-mail the corresponding author ; Nils Tofta ; ntoft@vet.dtu.dk" class="auth_mail" title="E-mail the corresponding author ; Anna Camilla Birkegå ; rda ; acbir@vet.dtu.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pooling ; Antimicrobial resistance gene ; Pig ; High-throughput qPCR ; Sample size ; Herd level
- 刊名：Veterinary Microbiology
- 出版年：2016
- 出版时间：30 June 2016
- 年：2016
- 卷：189
- 期：Complete
- 页码：46-51
- 全文大小：1234 K
文摘
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Five samples should be pooled when quantifying herd average of AMR genes.
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Good agreement between repeated pooling of the same individual samples is observed.
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A less time consuming pooling methods is proposed.
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Good agreement is observed between different pooling methods.