Structural organisation and chromosomal mapping of the human Id-3 gene
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- 作者：Deed ; Richard W. ; Hirose ; Tohru ; Mitchell ; Erika L.D. ; Santibanez-Koref ; Mauro F. ; Norton ; John D.
- 关键词：(Helix-loop-helix ; transcription factor ; negative regulator ; evolutionary conservation ; human gene structure ; tumor cell ; chromosome translocation)
- 刊名：Gene
- 出版年：1994
- 出版时间：December 30, 1994
- 年：1994
- 卷：151
- 期：1-2
- 页码：309-314
- 全文大小：519 K
文摘
The helix-loop-helix (HLH) family of transcription factors plays a central role in the regulation of cell growth, differentiation and tumourigenesis. Members of the Id (inhibitor of DNA binding) class of these nuclear proteins are able to heterodimerise with and thereby antagonise the functions of other transcription factors of this family. We report here on the genomic organisation of the human Id3 (HLH 1R21/heir1) gene. Comparison with the two other mammalianId genes, Id1 and Id2, reveals a highly conserved protein coding gene organisation consistent with evolution from a common, ancestral Id-like gene. In addition, by using a yeast artificial chromosome (YAC) clone of Id3, we have finescale mapped the gene to chromosome band 1p36.1 by fluorescence in situ hybridisation (FISH) and, using the same FISH technique, we have detected heterogeneity in tumour-associated 1p36 chromosome translocations.