Structure of the ArsI C-As Lyase: Insights into the Mechanism of Degradation of Organoarsenical Herbicides and Growth Promoters
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- 作者：Venkadesh Sarkarai Nadar1 ; vsarkara@fiu.edu" class="auth_mail" title="E-mail the corresponding author ; Masafumi Yoshinaga1 ; 2 ; myoshina@fiu.edu" class="auth_mail" title="E-mail the corresponding author ; Shashank S. Pawitwar1 ; Palani Kandavelu3 ; Banumathi Sankaran4 ; Barry P. Rosen1 ; brosen@fiu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：MSMA ; monosodium methylarsenate ; MAs(V) ; methylarsenate ; Rox ; roxarsone (4-hydroxy-3-nitrophenylarsonic acid) ; As(III) ; inorganic arsenite ; As(V) ; inorganic arsenate ; MAs(III) ; methylarsenite ; βME ; β-mercaptoethanol ; Ni-SAD ; Ni-single-wavelength anomalous diffraction ; APS ; advanced photon source
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：5 June 2016
- 年：2016
- 卷：428
- 期：11
- 页码：2462-2473
- 全文大小：1477 K
文摘
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Arsenic is the most pervasive environmental toxin.
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ArsI is a microbial non-heme, ferrous-dependent dioxygenase that detoxifies organoarsenical herbicides and growth promoters.
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The structure of ArsI shows a novel organoarsenical binding site.
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A loop-gating mechanism is proposed for substrate access to the catalytic site.