Ring crystals of oligonucleotides: Growth stages and X-ray diffraction studies

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• 作者：Pradeep K. M ; al ; Arun Richard Ch ; rasekaran ; Bharath Raj Madhanagopal ; Sarkarai Venkadesh ; Namasivayam Gautham
• 关键词：A1. Biocrystallization ; A1. Crystal morphology ; A1. Crystal structure ; A1. X-ray diffraction
• 刊名：Journal of Crystal Growth
• 出版年：2012
• 出版时间：1 September, 2012
• 年：2012
• 卷：354
• 期：1
• 页码：20-26
• 全文大小：616 K

文摘

The hexamer duplex d(CACGCG).d(CGCGTG) produces unusual hexagonal ring shaped crystals when crystallized in the presence of cobalt hexammine chloride. Observation of the growth of these crystals over a period of about 6 days indicated that this morphology resulted from radial dissolution of the crystal from the center of the well-formed hexagonal plates. X-ray diffraction studies were carried out for crystals of four stages in the ring crystal morphology. The structure of the DNA in all four crystals was the Z type DNA double helix. The packing mode of the Z DNA duplex in all four crystal structures was that of the ¡®spermine¡¯ form. The crystal morphology changes from hexagonal plates to hexagonal rings without any apparent change in the molecular structure of the oligonucleotide or helical packing of the duplexes.