Overexpression of Δ¹-pyrroline-5-carboxylate synthetase increases proline production and confers salt tolerance in transgenic potato plants

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• 作者：Aï ; da Hmida-Sayari ; Radhia Gargouri-Bouzid ; Amira Bidani ; Leï ; la Jaoua ; Arnould Savouré ; and Samir Jaoua
• 关键词：Δ ; ¹-Pyrroline-5-carboxylate synthetase (P5CS) ; Potato ; Proline ; Salt tolerance ; Solanum tuberosum ; Osmoregulation
• 刊名：Plant Science
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：169
• 期：4
• 页码：746-752
• 全文大小：260 K

文摘

Proline (Pro) is known as compatible osmolyte accumulated by plant cells in response to salt and drought stresses. It is supposed to be an osmoprotectant involved in the protection of cellular structures under osmotic stress. Therefore, in an attempt to increase salt tolerance in potato, a pyrroline-5-carboxylate synthetase (P5CS) cDNA from Arabidopsis thaliana was transferred to potato plants via Agrobacterium-mediated transformation. This enzyme is responsible for conversion of glutamate to Δ¹-pyrroline-5-carboxylate that is reduced to Pro. The resulting transgenic potato plants showed an important increase in Pro production levels compared to non-transgenics. This Pro accumulation was particularly enhanced in the presence of salt up to 100 mM NaCl. The transgenic potato plants also showed an improved tolerance to salinity through a much less altered tuber yield and weight compared to the non-transgenic ones.