When can real-time quantitative RT-PCR effectively define molecular relapse in acute promyelocytic leukemia patients? (Results of the French Belgian Swiss APL Group)
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- 作者：Bruno Cassinat ; Sté ; phane de Botton ; Charikleia Kelaidi ; Lionel Ades ; Fabien Zassadowski ; Isabelle Guillemot ; Marie-Helene Schlageter ; Emmanuel Raffoux ; Jean-Luc Harousseau ; Olivier Legr ; Martine
- 关键词：APL ; RQ-PCR ; Relapse ; Minimal residual disease ; Prognosis
- 刊名：Leukemia Research
- 出版年：2009
- 出版时间：September 2009
- 年：2009
- 卷：33
- 期：9
- 页码：1178-1182
- 全文大小：450 K
文摘
10–20 % of APL patients relapse and the challenge remains to early identify these patients to improve survival rate. We report PML-RARα transcript detection by RQ-PCR in 260 consecutive APL patients (n = 970 samples). 223 patients with samples of sufficient RNA quality to demonstrate they reached molecular remission were monitored for MRD. During follow-up, 38 of these patients were tested positive for PML-RARα mRNA. 13 out of the 38 patients (34 % ) effectively developed hematological relapse. In the first positive sample, specific PML-RARα NCN thresholds over which, or under which, patients could effectively be predicted to relapse or not, were identified and subsequently validated in a second cohort.