The epitopes of antibodies were successfully mapped with the novel multiparametric imaging platform.
Biotinylated peptides were not chemically synthesized but manufactured recombinantly in <em>Escherichia coliem>.
Incubating crude <em>E. coliem> lysates with streptavidin coupled microbeads were enough for peptide immobilization.
The analysis can be completed within three weeks starting from the delivery of the oligonucleotides for cloning.