Volume regulation of murine T lymphocytes relies on voltage-dependent and two-pore domain potassium channels

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• 作者：Nicole Bobak^a ; ^b ; Stefan Bittner^a ; ^b ; Joseph Andronic^c ; Susanne Hartmann^c ; Friederike Mü ; hlpfordt^c ; Tilman Schneider-Hohendorf^a ; ^b ; Karen Wolf^c ; Carsten Schmelter^c ; Kerstin Gö ; bel^a ; ^b ; Patrick Meuth^d ; Heiko Zimmermann^e ; ^f ; Frank Dö ; ring^g ; Erhard Wischmeyer^g ; Thomas Budde^d ; Heinz Wiendl^a ; ^b ; Sven G. Meuth^a ; ^b ; ¹ ; ^{sven.meuth@ukmuenster.de"" rel=""nofollow} ; Vladimir L. Sukhorukov^c ; ¹
• 关键词：Cell volumetry ; Regulatory volume decrease ; Osmotic stress ; T lymphocyte ; Potassium channel ; K_2P channels
• 刊名：Biochimica et Biophysica Acta (BBA)/Biomembranes
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：1808
• 期：8
• 页码：2036-2044
• 全文大小：1008 K

文摘

A variety of ion channels are supposed to orchestrate the homoeostatic volume regulation in T lymphocytes. However, the relative contribution of different potassium channels to the osmotic volume regulation and in particular to the regulatory volume decrease (RVD) in T cells is far from clear. This study explores a putative role of the newly identified K_2P channels (TASK1, TASK2, TASK3 and TRESK) along with the voltage-gated potassium channel K_V1.3 and the calcium-activated potassium channel K_Ca3.1 in the RVD of murine T lymphocytes, using genetic and pharmacological approaches. K_2P channel knockouts exerted profound effects on the osmotic properties of murine T lymphocytes, as revealed by reduced water and RVD-related solute permeabilities. Moreover, both genetic and pharmacological data proved a key role of K_V1.3 and TASK2 channels in the RVD of murine T cells exposed to hypotonic saline. Our experiments demonstrate a leading role of potassium channels in the osmoregulation of T lymphocytes under different conditions. In summary, the present study sheds new light on the complex and partially redundant network of potassium channels involved in the basic physiological process of the cellular volume homeostasis and extends the repertoire of potassium channels by the family of K_2P channels.