Influenza vaccine: Development of a novel intranasal and subcutaneous recombinant adjuvant

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• 作者：R. Segura-Vel¨¢zquez ; J. Cervantes ; E. Acosta ; I. S¨¢nchez-Betancourt ; N. Villalobos ; L.F. Rodarte ; M. Restelli ; G. Fragoso ; E. Sciutto
• 关键词：Adjuvant ; Phages ; Influenza ; Intranasal immunization ; Vaccination
• 刊名：Vaccine
• 出版年：2013
• 出版时间：20 August, 2013
• 年：2013
• 卷：31
• 期：37
• 页码：4009-4016
• 全文大小：1045 K

文摘

The synthetic peptide GK-1, derived from Taenia crassiceps, enhances the protection induced by human influenza vaccine in both young and aged mice. Herein, the adjuvant properties of GK-1 fused to the pVIII protein of a heat-inactivated phagemid vector (FGK1) when co-administered with the influenza vaccine were assessed, to evaluate its feasibility as a low-cost adjuvant. In mice, FGK1 significantly increased the expected IgG and IgA anti-influenza antibody levels both in sera and in bronchoalveolar fluids when intranasally or subcutaneously co-administered with influenza vaccine. Single-dose pig co-immunization with FGK1 and influenza vaccine induced serum levels of IgG anti-influenza antibodies similar to those elicited by a two-dose immunization with the influenza vaccine alone. Preclinical evaluation of FGK1 with the influenza vaccine is currently in progress, in order to recommend its use for veterinary purposes.