Recombinant glycoproteins: The impact of cell lines and culture conditions on the generation of protein species
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- 作者：Julia Rosenlö ; chera ; b ; Grit Sandiga ; Christoph Kannichtc ; Vé ; ronique Blanchardd ; Stefan O. Reinkea ; Stephan Hinderlicha ; stephan.hinderlich@beuth-hochschule.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：A1AT ; alpha-1-antitrypsin ; ADCC ; antibody-dependent cellular cytotoxicity ; AEM ; adenovirus expression medium ; CHO ; Chinese hamster ovary ; sDHB ; super-dihydroxybenzoic acid ; Fc ; fragment crystallizable ; GalNAc ; N-acetylgalactosamine ; GlcNAc ; N-acetylglucosamine ; HEK ; human embryonic kidney ; Neu5Ac ; N-acetylneuraminic acid
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：16 February 2016
- 年：2016
- 卷：134
- 期：Complete
- 页码：85-92
- 全文大小：643 K
文摘
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The diversity of the proteome is increased remarkably by protein glycosylation.
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N-glycans of two glycoproteins underwent structural analysis by mass spectrometry.
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A calculation of potential glycoforms for both glycoproteins was presented.
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The definitions of “glycoforms” and “protein species” were combined to a new term.