Step changes in leaf oil accumulation via iterative metabolic engineering

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• 作者：Thomas Vanhercke^a ; ¹ ; ^{Thomas.Vanhercke@csiro.au} ; Uday K. Divi^a ; ¹ ; Anna El Tahchy^a ; Qing Liu^a ; Madeline Mitchell^a ; Matthew C. Taylor^b ; Peter J. Eastmond^c ; Fiona Bryant^c ; Anna Mechanicos^a ; Cheryl Blundell^a ; Yao Zhi^a ; ^d ; Srinivas Belide^a ; Pushkar Shrestha^a ; Xue-Rong Zhou^a ; Jean-Philippe Ral^a ; Rosemary G. White^a ; Allan Green^a ; Surinder P. Singh^a ; James R. Petrie^a
• 关键词：DAG ; diacylglycerol ; DAS ; days after sowing ; DGDG ; digalactosyldiacylglycerol ; DW ; dry weight ; MGDG ; monogalactosyldiacylglycerol ; PC ; phosphatidylcholine ; SDP1 ; Sugar-dependent lipase1 ; TAG ; triacylglycerol ; VLCFA ; very long chain fatty acids
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：237-246
• 全文大小：1058 K
• 卷排序：39

文摘

TAG accumulation in metabolically engineered tobacco leaves is limited by a futile cycle. Iterative metabolic engineering results in the accumulation of >30% TAG in tobacco leaf tissue. Transgenic leaves resemble oilseed sink tissues and exhibit major lipid & carbon metabolic changes. TAG levels achieved in leaves now surpass those of the oilseed crop soybean.