Structure-based analysis of Bacilli and plasmid dihydrofolate reductase evolution

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• 作者：Mona Alotaibi^a ; ^b ; ^{monaotaibi@ksu.edu.sa} ; Ben Delos Reyes^a ; Tin Le^a ; Phuong Luong^a ; Faramarz Valafar^c ; ^{faramarz@mail.sdsu.edu} ; Robert P. Metzger^a ; ^{rmetzger@mail.sdsu.edu} ; Gary B. Fogel^d ; ^{gfogel@natural-selection.com} ; David Hecht^a ; ^e ; ^{dhecht@swccd.edu}
• 关键词：Dihydrofolate Reductase ; DHFR ; Structure-based Alignment ; Phylogeny ; Evolution ; Plasmid evolution ; Horizontal Gene Transfer ; Network of Coupled Protein Motion
• 刊名：Journal of Molecular Graphics and Modelling
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：71
• 期：Complete
• 页码：135-153
• 全文大小：5260 K
• 卷排序：71

文摘

A structure-based alignment of Plasmodium DHFR from 350 Bacilli and plasmid DHFR sequences was performed. The degree of conservation or variability was determined at each site in the structure-based alignment. Phylogenic trees were generated and evidence for horizontal gene transfer was identified. Active-site residues appear to be more highly conserved in plasmid DHFR sequences than chromosomal DHFR sequences. With respect to concerted protein motions, there is greater conservation in plasmid versus chromosomal proteins.