Different sealers demonstrated different antimicrobial activity against E. faecalis.
Positive antimicrobial activity of freshly mixed sealers against E. faecalis up to 24 h.
Antimicrobial activity was lost as the material set with no inhibitory effect for 2–7 d.
Standardizing in vitro tests for antimicrobial activity of endodontic sealers is needed.
More studies are needed to evaluate sealers' effect against multiple endodontic pathogens.