Standardization of sequence based identification is proposed.
DNA barcoding of pathogenic fungi in clinical settings is discussed.
The curated ISHAM-ITS reference database is presented.
The value of ITS region as fungal barcode is reviewed at intra/interspecies level.
The database is accessible at http://www.isham.org/ or http://its.mycologylab.org.
For some fungi the use of secondary barcodes may be necessary.