Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)

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• 作者：Krzysztof Mikołajczak ; Piotr Ogrodowicz ; Maria Surma ; Tadeusz Adamski ; Anetta Kuczyńska ; ^{akuc@igr.poznan.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：BC lines ; Gene annotation ; Semi-dwarf gene ; Single nucleotide polymorphism spring barley ; Marker selection ; Marker-assisted backcrossing ; Marker-assisted introgression ; DNA markers ; Selection in plant breeding
• 刊名：Electronic Journal of Biotechnology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：24
• 期：Complete
• 页码：9-11
• 全文大小：202 K

文摘

Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search against known genes is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized for SNP search in genes of predicted function of interest and associated gene ontology (GO).

Results

Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in backcrossing targeted on the selected gene.

Conclusions

BC₆ lines developed in this study can serve as a unique and adequate plant material to dissect the role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response to abiotic stress.