The use of proteases to remove protein-based residues from solid surfaces

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• 作者：Kevin Turner ; Marina Serantoni ; Angela Boyce ; Gary Walsh
• 关键词：Alcalase ; Protease ; Enzyme-cleaning ; Fouling ; Glass
• 刊名：Process Biochemistry
• 出版年：2005
• 出版时间：October 2005
• 年：2005
• 卷：40
• 期：10
• 页码：3377-3382
• 全文大小：302 K

文摘

The efficiency of the commercial proteolytic preparation ‘Alcalase’ in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by monitoring polypeptide/peptide/amino acid accumulation in the wash and by three-dimensional atomic force microscopy. The results indicate that treatment with enzyme at industrially realistic inclusion levels removes a very significant proportion of protein foulant and that post-cleaning residual enzyme activity on the glass surface can be easily removed or inactivated. Results are discussed in the context of their potential application in the bioprocess sector.